Laser-Speckle Contrast Imaging

The RFLSI-ZW laser speckle imaging system is a great tool for microcirculation research based on laser speckle contrast imaging technology (LSCI). With the advanced optical design and improved image processing algorithm, RFLSI-ZW shows greater performance in imaging field size, image quality, full-field frame rate and optical resolution, and provides a powerful and efficient means for human and animal tissue blood flow measurement.

Overview

The LSCI technology advantages are its non-contact, high frame-rate, high spatial resolution. These features are particularly useful to observe and record blood perfusion of any exposed tissues or organs for microcirculation study or pre-clinical research topics such as ischemic stroke, lower limbs, mesentery, etc. Multi-Output includes blood perfusion images and videos (500+ million pixels), quantified data for perfusion unit and vessel diameter.

Trusted by over 100 renowned research institutions

Since 2019, this imaging system has been adopted by more than 100 colleges, universities and research institutes worldwide such as Stanford University School of Medicine, University of Manchester, UC Davis, Yale University and many more, where it has contributed to publishing more than 100 reputed research papers in magazines like Nature communications, Blood, Diabetes, and Theranostic.

Highlights

  • Image any exposed tissue (skin or surgically exposed tissues) and species.

  • Non-contact, non-contrast agent depending measurement.

  • Best optical resolution of 3.9 μm/pixel, providing more detailed tissue structures.

  • Max frame rate (full field) up to 100 fps, acquiring real-time changes in larger areas. 

  • The built-in CMOS global shutter camera can achieve faster data acquisition and processing.

  • Motorised 10x optical zoom and auto focus

  • Image size ranges from 0.57×0.75 to 22.5×30 cm² in all-in-one imager, covering multiple research applications.

  • Fast auto and fine manual focus, improving focus efficiency and accuracy on various tissues.

  • Optimal lens assembly, filtering the ambient and reflecting light.

  • Class 1 of measurement and indicating lasers, safe to use without eye protection System.

  • Laser stability hardware for the ultimate in reliable and consistent measurement over minutes, hours and days.

  • Calibration with calibration box. Self-calibration is possible at any time to keep the equipment in optimal working condition.

  • Trigger In/Out BNC connections for communication with external devices.

  • Unlimited installation of analysis software for PC.

Applications

  • Cerebral blood perfusion monitoring
  • MCAO model assessment
  • Cortical spreading depression observation
  • Hind-limb ischemia research
  • Skin burn/skin flap transplantation
  • Organ microcirculation observation
  • Skin allergies
  • Septic Shock
  • Chicken Chorioallantoic Membrane Assay
  • Diabetic Foot